Curriculum Vitae

PERSONAL DETAILS
ACADEMIC QUALIFICATION
(Qualification),(Institution)
  • Doctor of Philosophy in Nanobiotechnology, UPM, Malaysia
  • Bachelor of Science (Hons) Microbiology, UPM, Malaysia
PROFESSIONAL MEMBERSHIP
(Organisation),(Role),(Duration),(Level)
  • European Society for Nanomedicine (ESNAM), Life member, Since 2018 (International)
  • BactiVac Network, Member, Since 2018 (International)
  • Malaysia Society for Biochemistry and Molecular Biology (MSBMB) , Member, 2012 to 2021 (National)
AREAS OF EXPERTISE
(Area)
  • Molecular virology (hepatitis B virus, influenza virus, turnip yellow mosaic virus, bacteriophage) (Viral protein expression and purification; Capsid symmetry and virus architecture; Capsid assembly and stability)
  • Drugs and gene delivery; Vaccine delivery and development. (Virus-like particles, nanoparticles, cell-targeting, cancers)
PUBLICATIONS
(Title of publication in APA style)
Book Chapter
2022
  • Wong C.L., Yong C.Y., Lee K.W. (2022) Phage T7 as a Potential Platform for Vaccine Development. In: Thomas S. (eds) Vaccine Design. Methods in Molecular Biology, vol 2412. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1892-9_5
  • Khalaj-Hedayati, A., Chua, C. L. L., Smooker, P., & Lee, K. W. (2022). Universal influenza vaccine technologies and recombinant virosome production. https://doi.org/10.1016/BS.MIM.2022.04.001
Article in Journals
2023
  • Tan FH, Ng JF, Mohamed Alitheen NB, Muhamad A, Yong CY, Lee KW. A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation. J Virol Methods. 2023 Sep;319:114771. doi: 10.1016/j.jviromet.2023.114771. Epub 2023 Jul 10. PMID: 37437780. (ISI-Indexed)
     
2021
  • Tan, F. H., Ng, J. F., Alitheen, N. B., Wong, C. L., Yong, C. Y., Lee, K. W., 2021. Recombinant Turnip Yellow Mosaic Virus Coat Protein as a Potential Nanocarrier. J. Appl. Microbiol. (ISI-Indexed)
2020
  • Khalaj-Hedayati, A., Chua, C. L. L., Smooker, P., Lee, K. W., 2020. Nanoparticles in influenza subunit vaccine development: Immunogenicity enhancement. Influenza Other Respir. Viruses. 14, 92-101. (ISI-Indexed)
     
2013
  • Yoon, K. Y., Tan, W. S., Tey, B. T., Lee, K. W., Ho, K. L., 2013. Native agarose gel electrophoresis and electroelution: A fast and cost effective method to separate the small and large Hepatitis B nucleocapsids. Electrophoresis 34, 244-253. (ISI-Indexed)
     
2012
  • Lee, K. W., Tey, B. T., Ho, K. L., Tan, W. S., 2012. Delivery of chimeric hepatitis B core particles into liver cells. J. Appl. Microbiol. 112. 119-131. (ISI-Indexed)
     
  • Lee, K. W., Tey, B. T., Ho, K. L., Tejo, B. A., 2012. Nano-glue: An alternative way to display cell-internalizing peptide (CIP) at the spikes of hepatitis B virus core nanoparticles for cell-targeting delivery. Mol. Pharm. 9, 2415-2423. (ISI-Indexed)
     
2008
  • Lee, K. W. and Tan, W. S., 2008. Recombinant hepatitis B virus core particles: Association, dissociation and encapsidation of green fluorescent protein. J. Virol. Methods 151. 172-180. (ISI-Indexed)
     
Mass Media(Newspaper,Radio,TV,Popular Magazine)
2017
  • Lee, K. W. and Chua, L. L., 2017. Understanding vaccine (解读疫苗). Oriental Daily News, Malaysia. 20th Feb 2017: L7. Print (in Chinese).
     
Digital Media
2024
  • Lee, K. W., 2024. Purification of virus-like particles. GEN Genetic Engineering & Biotechnology News by Mary Ann Liebert, Inc., United States. 14th Feb 2024: Bioprocessing. Digital media.
     
RESEARCH PROJECT
(Project title),(Role),(Duration),(Level)
  • New vaccination technology via skin absorption: Elucidation of skin penetration and immunogenicity profiles of an antigen model, Project Leader, 2022 to 2025 (National)
  • Investigation on antiviral effects and immune regulatory activity of Lactobacillus casei & Lactobacillus acidophilus live culture strains and probiotic secreted components against respiratory syncytial virus and rotavirus in vitro, Project Leader, 2022 to 2023 (Private)
  • Serial monitoring of SARS-CoV-2 in the saliva of patients (Project extension), Co-researcher, 2021 to 2022 (National)
  • Serial monitoring of SARS-CoV-2 in the saliva of patients., Co-researcher, 2020 to 2021 (National)
  • Design and Innovation of a Prototype of Versatile Biosensor Using Recombinant IgM Binding Protein (FcMR) , Co-researcher, 2020 to 2021 (Taylor's University)
  • Display of conserved peptide from stalk region of Influenza hemagglutinin on Hepatitis B virus capsid for universal vaccine development via Nano-glue bioconjugation, Project Leader, 2018 to 2019 (Taylor's University)
  • SYNTHESIS AND CHARACTERIZATION OF NANOPARTICLES FROM PLANT VIRUSES FOR DRUG AND GENE DELIVERY, Project Leader, 2013 to 2016 (National)
  • Display of the conserved antigenic epitopes of influenza virus and cell targeting peptide on HBV nanoparticles., Project Leader, 2013 to 2016 (Taylor's University)
CONSULTANCY PROJECT
(Project title),(Organisation),(Duration),(Role),(Level)
  • Evaluation of bactericidal activity of cosmetics and personal care formulations., Winnox Cosmeceutics Sdn Bhd, 01/09/2022 to 30/06/2023, Consultant  (Private/Industrial Sector)
  • Evaluation of antibiotic susceptibility profile of probiotics. , GN Neutraceuticals Sdn. Bhd., 14/06/2022 to 09/09/2022, Consultant  (Private/Industrial Sector)
  • Evaluation of bactericidal activity of cosmetics and personal care formulations. , Winnox Cosmeceutics Sdn Bhd, 10/05/2022 to 03/08/2022, Consultant  (Private/Industrial Sector)
SUPERVISION
(Title of Thesis),(Name of Candidates),(Academic Level),(Role)
COMPLETED
  • Surface Functionalization of Turnip Yellow Mosaic Virus (TYMC) Capsid By Chemical Conjugation of Fluorescein And Genetical Fusion of HIV-1 TAT Peptide., KONG JIA CHEN  (Main Supervisor)
  • PRODUCTION AND CHARACTERIZATION OF INFLUENZA RECOMBINANT VIROSOME USING CONSERVED HEMAGGLUTININ PEPTIDE AND HEPATITIS B CORE ANTIGEN (HBCAG), ATIN KHALAJHEDAYATI  (Main Supervisor)
  • PRODUCTION AND MODIFICATION OF RECOMBINANT TURNIP YELLOW MOSAIC VIRUS COAT (TYMVC) NANOPARTICLE FOR TARGETED DELIVERY INTO PC3 HUMAN PROSTATE CANCER CELLS., TAN FOO HOU  (Main Supervisor)
ONGOING
  • HUMAIRA SIDDIQUI  (Main Supervisor)
  • PAO CHAN  (Main Supervisor)
  • AMEERAH MONTREE BINTI MUHAMMAD  (Main Supervisor)
  • MOHAMAD ALIF BIN ISMAIL  (Main Supervisor)
PRESENTATION
(Title),(Event),(Date Presented),(Organiser),(Level)
INVITED SEMINAR
  • Aseptic technique and the isolation of microorganisms., Microbiology Workshop , 24/05/2017 to 24/05/2017, Universiti Teknologi MARA (UiTM), Sungai Buloh campus, Sungai Buloh, Selangor, Malaysia. (National)
  • Virus-like particle (VLP) - A platform for peptide presentation via genetic & chemical modifications., Biotechnology and Biological Sciences Seminar, 21/03/2014 to 21/03/2014, School of Applied Sciences, RMIT University.  (International)
ORAL PRESENTER
  • Molecular Display on Virus/Virus-like Nanomaterials Via Chemical Approach, 1st International Conference on Applied Chemistry (1 ICAC), dated 14-17 march 2015, 14/03/2015 to 17/03/2015, Sohag University and International Society of Applied Chemistry and Community Development (http://www.sohag-univ.edu.eg/isac-chem/) (International)
  • Targeting HeLa cells using hepatitis B virus-like particle (HBVLP) decorated with nanoglue-cell-internalizing peptides. , 5th BBBB International Conference, from drug discovery and formulation strategies to pharmacokinetics-pharmacodynamics., 26/09/2013 to 28/09/2013, University of Athens (International)
PLEANARY/KEYNOTE SPEAKER
  • Molecular display on virus/virus-like nanomaterials via chemical approach. In 1st International conference on applied chemistry., 1st International conference on applied chemistry, 14/03/2016 to 17/03/2016, Sohag University and International Society of Applied Chemistry and Community Development (ISAC-CD) (International)
INTELLECTUAL PROPERTY RIGHT (IPR)
(Product Name),(Type),(Level)
  • A Protein Purification Method, Patent (National)
AWARDS AND STEWARDSHIP
(Name of Award/Stewardship),(Award Category),(Awarding Institution),(Year Awarded),(Type of Award)
  • SILVER AWARD: International University Carnival on E-Learning (IUCEL 2023) "Empowering Society Through Education 5.0"., Education, Universiti Malaysia Pahang Al-Sultan Abdullah, 2023 (Creativity)
  • FAOBMB Travel Fellowship 2018, Special, Chulalongkorn University, Thailand, 2018 (Fellowship)
  • Employee Education Subsidy Scheme for Postgraduate Certificate in Higher Education Teaching and Learning, 2012-2014 (Taylor s University, Malaysia), Education, Taylor's University, 2012 (Scholarship )
  • Gold Medal Award-the Invention, Research and Innovation Exhibition 2008 (PRPI 08). , Exhibition/Invention, Universiti Putra Malaysia (UPM), 2008 (Creativity)